DOG GENETICS

Canine Genetic Linkage Map

http://www.ackchf.org

The canine linkage map is the principal tool for gaining unbiased genetic access to causal pathways underpinning diseases in the dog. Previously published maps have consisted of fewer than 500 markers. The linkage map presented here is comprehensive, and is based on pedigree resources with greater mapping power than the human CEPH.

Browse the Genome By Linkage

Karyotype image adapted from Ensembl

Download: Linkage Map File [xls]

Previous Builds

Build Phase	Date	Markers
Phase 5	July 2006	2488
Phase 4	January 2006 v1.2	1795
	January 2006 (CAP Grant)	1795
Phase 3	December 2005 (v1.1)	1417
Phase 2	June 2005 - v1.0	833
	June 2005 (CAP Grant)	833
Phase 1	January 2005 (PAG)	702

Protocols

M13 Tailed Primer Protocol

PCR Conditions

Thermocycling Program

Post PCR

Linkage Map Statistics

Map Build	September 2008 (Submitted)
Total Markers Mapped	3075
Total Genetic Length	2085 cM
Average spacing	.7 +/- .9 cM
Gaps <= 1 cM	74%
Gaps < 5 cM	98%
Total DNAs	281
Total Avg Inf Meioses	201 +/- 91

Marker Information

Primer3 Parameters

Product Size:	100-400 bp
Primer Size (min)	19
Primer Size (opt)	20
Primer Size (max)	22
Tm (min)	59
Tm (opt)	62
Tm (max)	64

Comprehensive Microsatellite Markers

For a comprehensive set of 32,135 mined microsatellite markers using the same design parameters as the linkage map loci, see DOGSET