Parentage/Genetic Marker ReportQuick Summary
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A DNA profile of species-specific microsatellite markers is obtained from the offspring, potential dam, and potential sire(s), and parentage analysis is performed using this data. In this process, a computer program compares the DNA profile of the offspring to the profiles of the presumed parents. Candidate sires and dams may either qualify or be excluded as parents based on whether they share microsatellite marker alleles with the offspring. A parentage analyst then reviews the results and sends the final report. If a listed parent or parents are excluded, additional analysis is performed, including retesting of samples and the possible use of additional DNA markers to confirm the exclusion.
You can read more about the process of DNA parentage analysis HERE.
Sample Collection
Most goat DNA tests offered by the VGL are carried out using 20-30 hairs with roots.
Hair root from coarse, longer hair often found over withers, chest, rump, tail, back of hind leg, poll, or fetlock is the preferred sample type for goats.
Instructions
1. Clean (use comb if possible) by removing all loose hair and foreign matter.
2. Use fingers or pliers to grasp approximately 8-10 hairs close to the skin and pull. Pull (do NOT cut) hair strands. Examine the end of hair strands for presence of root bulbs. Hair roots are necessary for DNA testing. If the majority of hair strands lack the root bulbs, discard hair and start again.
3. Repeat until you have approximately 20-30 hairs with root follicles attached.
4. Place the 20-30 hairs with root follicles attached in the envelope and seal with the animal’s ID written on the envelope.
5. Repeat steps 1-4 for each additional animal being sampled.
Note:
- Hair should be dry.
- If hair has excess dirt and debris, please brush out if possible before pulling hairs for sample.
- Do not cut the hair! The roots contain the DNA for testing.
- When sampling several animals in the same session, make sure that there are no hair strands in your hands to reduce the possibility of sample contamination. Clean hands and/or pliers if possible.
Examples
Sampling goat chest hair:
Sampling goat rump hair:
Sampling technique using pliers:
In goats, the following microsatellite markers are reported:
CSRD247
MAF65
McM527
OarFCB20
SPS113
SRCRSP23
SRCRSP5
SRCRSP8
HSC
ILSTS008
ILSTS19
ILSTS87
INRA005
INRA006
INRA023
INRA063