Canine Leukocyte Adhesion Deficiency (CLAD) in German Shepherd Dogs

Quick Summary

Canine leukocyte adhesion deficiency, Type III (CLAD-Type III) is an inherited blood disorder affecting German Shepherd Dogs. It is characterized by abnormal blood clotting and immune system functions.

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Phenotype: Affected German Shepherd Dogs have abnormal blood clotting and immune system functions and may present with lameness, recurrent skin infections, bone infections, and gingivitis. Although some dogs can live for years with this condition, most affected dogs die early.

Mode of Inheritance: Autosomal recessive

Alleles: N = Normal, CLADgs = Canine leukocyte adhesion deficiency (German Shepherd Dog variant)

Breeds appropriate for testing: German Shepherd Dog

Explanation of Results:

  • Dogs with N/N genotype will not have canine leukocyte adhesion deficiency and cannot transmit this variant to their offspring.
  • Dogs with N/CLADgs genotype will not have canine leukocyte adhesion deficiency, but are carriers. They will transmit this CLAD variant to 50% of their offspring. Matings between two carriers are predicted to produce 25% canine leukocyte adhesion deficiency-affected puppies.
  • Dogs with CLADgs/CLADgs genotype will have canine leukocyte adhesion deficiency, a potentially fatal condition.

Results of this test can be submitted to the OFA (Orthopedic Foundation for Animals)

Turnaround Time
At least 15 business days; may be delayed beyond 15 business days if sample requires additional testing, or a new sample is requested.
Price

$55 single test per animal ($5 discount on 3 or more dogs)
$25 as additional health test on same animal

Sample Collection

Dog DNA tests are carried out using cells brushed from your dog's cheeks and gums. The preferred cytology brushes are sent to you by mail, or you may provide your own brushes. For accepted alternative brushes, click here

We recommend waiting until puppies are at least three weeks old before testing.

 

Dog having its cheeks and gums brushed for DNA samples
Cheek and gum brushing technique for canine DNA sample collection

Step-By-Step:

  1. Make sure the dog has not had anything to eat or drink for at least 1 hour prior to collecting sample.
  2. When swabbing puppies, isolate each puppy from the mother, littermates and any shared toys for 1 hour prior to swabbing. Puppies should not have nursed or eaten for 1 hour prior to collecting sample.
  3. If collecting samples from more than one dog, make sure to sample one dog at a time and wash your hands before swabbing another dog.
  4. Label brush sleeve with name or ID of dog to be sampled.
  5. Open brush sleeve by arrow and remove one brush by its handle.
  6. Place bristle head between the dog’s gums and cheek and press lightly on the outside of the cheek while rubbing or rotating the brush back and forth for 15 seconds.
  7. Wave the brush in the air for 20 seconds to air dry.
  8. Insert brush back into sleeve.
  9. Repeat steps 5 - 8 for each unused brush in sleeve on a fresh area of cheek and gums. Make sure to use and return all brushes sent by the VGL. In most cases, it will be 3 brushes per dog. If using interdental gum brushes, please note that the VGL requires 4 brushes per dog and only moderate or wide interdental gum brushes are accepted.
  10. Do not seal brushes in sleeve.
  11. Place all samples in an envelope and return to the address provided.

ATTENTION:

  • Do not collect saliva/drool – the key to obtaining a good sample is getting cheek cells on the swab
  • Do not rub swab on the dog’s tongue or teeth – this will result in poor quality sample
  • Do not collect a sample from a puppy that has recently nursed – the mother’s genetic material can rub off on the puppy’s mouth and contaminate the sample
Additional Details

Canine leukocyte adhesion deficiency (CLAD) is an inherited blood disorder affecting Irish Setters and German Shepherd Dogs. The disease results from breed-specific mutations in genes that are integral to platelet and blood cell activity. Affected dogs have abnormal blood clotting and immune system functions. Affected German Shepherd Dogs and Irish Setters may present with lameness, recurrent skin infections including pyoderma (pus filled skin infections), furunculosis (boils) and ulceration, osteomyelitis (bone infections) and gingivitis. Although some dogs can live for years with this condition, most affected dogs die early from severe infection or bleeding from an accidental injury, or during a surgical procedure. Veterinarians should be informed of affected dogs prior to any surgical procedures.

CLAD-Type III (reported as CLADgs) in German Shepherd Dogs results from a 12bp insertion (c.1358_1359insAAGACGGCTGCC) in fermitin family member 3 gene (FERMT3). The disease is inherited in an autosomal recessive fashion, which means that males and females are equally affected and that two copies of the defective gene are needed to cause CLAD. Dogs with one normal and one affected gene (carriers) are normal and show no signs of disease.

Testing for CLAD assists clinicians with diagnosis of CLAD and helps breeders identify carriers among breeding stock to avoid producing affected dogs. Matings between carriers are expected to produce 25% of affected puppies.