Miniature American Shepherd Health Panel

Collie Eye Anomaly (CEA)

Collie Eye Anomaly (CEA), also known as choroidal hypoplasia, is a heritable disorder that can result in abnormal development of inner structures of the eye. This genetic variant is associated with CEA in multiple breeds.

Degenerative Myelopathy (DM)

Degenerative myelopathy (DM) is an inherited neurologic disorder of dogs characterized by gradual muscle wasting and loss of coordination typically beginning in the hind limbs. Testing is most appropriate for those breeds in which the clinical disease has been associated with the SOD1 allele.

Hereditary Cataracts (Australian Shepherd Type)

Cataracts (clouding of the lens of the eye) are a common cause of blindness in dogs. In Australian Shepherds, a genetic mutation causes hereditary cataracts, which may start forming after 2 years of age and show variable rate of progression and vision impairment.

Hyperuricosuria (HUU)

Hyperuricosuria is an inherited disorder characterized by elevated levels of uric acid in the urine that can lead to the formation of bladder/kidney stones.

Multidrug Sensitivity (MDR1)

Multidrug Sensitivity is caused by a deletion in the ABCB1 gene, also known as the multidrug resistance 1 (MDR1) gene, and is characterized by neurotoxicity following the use of certain common drugs. Dogs with two copies of MDR1 will exhibit multidrug sensitivity. Some dogs with 1 copy of MDR1 may also exhibit multidrug sensitivity.

Canine Multifocal Retinopathy 1 (CMR1)

Canine multifocal retinopathy 1 is an inherited eye disease characterized by areas of retinal detachment. The disease does not typically lead to blindness or vision deficits.

Chondrodystrophy (CDDY and IVDD) and Chondrodysplasia (CDPA)

Chondrodysplasia is a short-legged phenotype characteristic of many dog breeds. Chondrodystrophy, a separate mutation, also includes a short-legged phenotype as well as premature disc degeneration and increased susceptibility to disc herniation.

Sample Collection

Dog DNA tests are carried out using cells brushed from your dog's cheeks and gums. The preferred cytology brushes are sent to you by mail, or you may provide your own brushes. For accepted alternative brushes, click here

We recommend waiting until puppies are at least three weeks old before testing.

 

Dog having its cheeks and gums brushed for DNA samples
Cheek and gum brushing technique for canine DNA sample collection

Step-By-Step:

  1. Make sure the dog has not had anything to eat or drink for at least 1 hour prior to collecting sample.
  2. When swabbing puppies, isolate each puppy from the mother, littermates and any shared toys for 1 hour prior to swabbing. Puppies should not have nursed or eaten for 1 hour prior to collecting sample.
  3. If collecting samples from more than one dog, make sure to sample one dog at a time and wash your hands before swabbing another dog.
  4. Label brush sleeve with name or ID of dog to be sampled.
  5. Open brush sleeve by arrow and remove one brush by its handle.
  6. Place bristle head between the dog’s gums and cheek and press lightly on the outside of the cheek while rubbing or rotating the brush back and forth for 15 seconds.
  7. Wave the brush in the air for 20 seconds to air dry.
  8. Insert brush back into sleeve.
  9. Repeat steps 5 - 8 for each unused brush in sleeve on a fresh area of cheek and gums. Make sure to use and return all brushes sent by the VGL. In most cases, it will be 3 brushes per dog. If using interdental gum brushes, please note that the VGL requires 4 brushes per dog and only moderate or wide interdental gum brushes are accepted.
  10. Do not seal brushes in sleeve.
  11. Place all samples in an envelope and return to the address provided.

ATTENTION:

  • Do not collect saliva/drool – the key to obtaining a good sample is getting cheek cells on the swab
  • Do not rub swab on the dog’s tongue or teeth – this will result in poor quality sample
  • Do not collect a sample from a puppy that has recently nursed – the mother’s genetic material can rub off on the puppy’s mouth and contaminate the sample

Species

Dog

Type of Panel